Evaluation of actinobacillus pleuropneumoniae diagnostic tests using samples from experimentally infected pigs by giordana souza costa dr. Actinobacillus seminis isolated from cattle sciquest. Actinobacillus seminis as a cause of ram infertility in. Actinobacillus seminis infection in rams constitutes a spectrum of. Actinobacillus seminis definition of actinobacillus. Rapid and accurate identification of human isolates of pasteurella and related species by. Actinobacillus suis can cause similar disease and lesions but is not as contagious as actinobacillus pleuropneumoniae app. Semen obtained from infected rams yielded actinobacillus seminis in pure or mixed culture in five of the six clinically infected rams. Evidence from numerical taxonomic analysis and dnadna hybridization supports the proposal of new species in the genera actinobacillus and pasteurella. Experimentally infected with actinobacillus seminis and histophilus. Actinobacillus is a genus of gramnegative, nonmotile and nonsporeforming, oval to rodshaped bacteria occurring as parasites or pathogens in mammals, birds, and reptiles. Actinobacillusspecies and their role in animal disease. It has high morbidity and mortality rates and can cause severe economic losses to swine producers. Pdf this study reports the occurrence of sheep epididymitis and the isolation of actinobacillus seminis in the state of pernambuco, brazil.
Seminis disease guides provide descriptions and pictures of the more commonly found diseases and disorders worldwide by species. Actinobacillus seminis 2 pv actinobacillus succinogenes 2 pv actinobacillus suis 2 pv actinobacillus ureae 2 old name pasteurella ureae actinobaculum actinobaculum schaalii 2. Identification of an immunogenic protein of actinobacillus seminis that is present in microvesicles. Actinobacillus seminis infection in sheep in the republic. Actinobacillus minor actinobacillus porcinus actinobacillus. Pcr methods for rapid identification and characterization of.
Speciesspecific multiplex pcr for the diagnosis of brucella. December 2008 h a species abiotrophia 2 abiotrophia defectiva actinobacillus 2 actinobacillus capsulatus 2 actinobacillus equuli 2 actinobacillus hominis 2 actinobacillus lignieresii 2 actinobacillus pleuropneumoniae actinobacillus rossii 2 actinobacillus seminis 2 actinobacillus suis 2 actinobacillus urea actinobacillus. Actinobacillus suis an overview sciencedirect topics. Actinobacillus seminis, campylobacter fetus, escherichia coli, histophilus somni, mannheimia haemolytica, proteus vulgaris and gram. Infected with actinobacillus seminis and histophilus somni.
Method to grow actinobacillus pleuropneumoniae biofilm on. In the united states, app was first reported in 1957. Summary eighteen strains of actinobacillus seminis isolated from ovine semen samples and epididymitis were characterized according to their cultural and. Actinobacillus definition, any of several spherical or rodshaped, aerobic, parasitic bacteria of the genus actinobacillus, certain species of which, as a. Sep 10, 2010 actinobacillus actinomycetemcomitans is a typical cause of periodontitis but it may also be related to systemic infections and arterial plaques. Adobe acrobat xi pro was used to ocr the text and also for the merging and conversion to the final presentation pdf format. Actinobacillus seminis definition of actinobacillus seminis. The diagnosis of epididymitis by actinobacillus seminis was confirmed with association of the clinical findings. Actinobacillus capsulatus definition of actinobacillus. Infected rams were found to have abscessed testes and epididymi and.
Actinobacillus pleuropneumoniae app iowa state university. A genus of gramnegative, immotile and nonsporeforming, oval to rodshaped, often pleomorphic bacteria which occur as parasites or pathogens in mammals. Actinobacillus seminis is a gramnegative bacterium of the pasteurellaceae family that is involved in ovine epididymitis. Dear sir, in a paper submitted for publication in the australian veterinary journal, we have reported serological evidence of actinohocilluj seminis infection in 23 ewes from a border leicester flock. Pdf infectious epididymitis is considered a major cause of economic losses. Linear integrated circuits by ramakant gaikwad pdf opamps and linear integrated circuits on amazon. For each disease and disorder, you will find the common name, the cause, where it occurs, symptoms, and conditions necessary for development and control measures.
Actinobacillus seminis is a common cause of ovine epididymitis and ram infertility throughout the world 2, 6, 10, 21. Actinobacillus ross, actinobacillus equuli, and actinobacillus suis have been reported in cases of metritis, abortion, low number of viable pigs at birth, and decreased litter weights. Actinobacillus seminis as a cause of abortion in a uk sheep flock. Overview of actinobacillosis generalized conditions merck. Speciesspecific multiplex pcr for the diagnosis of. The isolates of a seminis showed similar phenotypic profiles and electrophoretic protein patterns to the type strain of a seminis but were distinct from histophilus ovis previously isolated from rams with epididymitis in scotland. The actinobacillus group shares many biological properties with the genus pasteurella. Deoxyribonucleic acid relationships of histophilus ovis. Infectious epididymitis is considered a major cause of economic losses for the sheep industry worldwide. A model for demonstrating the adhesion of actinobacillus seminis to epithelial cells. Find out information about actinobacillus lignieresi. Isolation of actinobacillus seminis from a goat with. Viral respiratory diseases pi3, rsv, adenovirus, reovirus, enzootic nasal tumors, opp ii.
Actinobacillus seminis in sheep, australian veterinary. Vallad botany and planting bell pepper capsicum annuum, solanaceae. Since then, the prevalence of ovine epididymitis due to a. Colonies are small, sticky and adherent to the agar surface. Actinobacillus actinobacillus actinomycetemcomitans refer to haemophilus actinomycetemcomitans. Actinobacillus definition of actinobacillus by the free. In commercial flocks semen examination is only worthwhile if there is some doubt as to ram fertility. Actinobacillus seminis is a pleomorphic, gramnegative bacillus that is a wellknown cause of epididymitis in australian rams baynes and simmons, 1960, and has been documented in the united states of america, south africa, new zealand, hungary, patagonia, the united kingdom, turkey, kidney, and spain. Twentyfour isolates of actinobacillus seminis were typed by pcr ribotyping, repetitive. Pdf actinobacillus seminis as a cause of abortion in a uk. It is associated with epididymitis of sheep references. The organisms are capable of infecting cattle and other domestic animals and, rarely, are associated with human infection. A study for the differentiation of actinobacillusseminis. It was not until 1960 that baynes and simmons 1960 reported another organism which could be included in the genus.
This bacterium is the causative agent of porcine pleuropneumonia, which is a highly contagious respiratory disease causing important economical losses to the worldwide pig industry. Identification of an immunogenic protein of actinobacillus. A model for demonstrating the adhesion of actinobacillus. Actinobacillus equuli definition of actinobacillus equuli. Abstract the organism commonly referred to as actinobacillus seminis is an accepted cause of suppurative epididymitis of rams worldwide, it has also been associated with polyarthritis, myocarditis and posthitis in sheep, and isolated from aborting ewes, goat does and their foetuses. Bacterial respiratory diseases mannheimia haemolytica biotype a, pasteurella trehalosi biotype t, mycoplasma ovipneumoniae iii. Actinobacillus pleuropneumoniae is a gramnegative bacterium and a member of the pasteurellaceae family. This was actinobacillus seminis isolated from epididymitis in three rams in australia. The aim of this study was to develop a multiplex pcr assay for simultaneous detection of brucella ovis, actinobacillus seminis, and histophilus. Clinical and pathological changes in rams experimentally infected. The most common causative agents of these infections are brucella ovis, actinobacillus seminis, and histophilus somni. Actinobacillus seminis in sheep actinobacillus seminis in sheep baynes, i. The etiologic agent, actinobacillus suis, is only now being studied in detail.
The main causative agents of ovine infectious epididymitis. On the other hand, one of the haemophilus agni strains studied hoerlein strain m65043 was included in the species actinobacillus seminis dna binding value, 91%. Actinobacillus seminis was isolated in pure and mixed culture from the urethra in two yearlings, one which was infected and one which was not, while bacilli were isolated from one uninfected ram. Read serological comparison between histophilus ovis, actinobacillus seminis and brucella ovis, australian veterinary journal on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Morphological, biochemical, antigenic, and cytochemical. Misdiagnosis of the disease as actinobacillus pleuropneumoniae app infection or other septicemias likely contributes to underreporting of the true prevalence of the disease.
The species is not recognised today and is almost certainly what we now term haemophilus somnus. Actinobacillus capsulatus synonyms, actinobacillus capsulatus pronunciation, actinobacillus capsulatus translation, english dictionary definition of actinobacillus capsulatus. Isolated actinobacillus actinomycetemcomitans from periodontitis patients releases leukotoxin which kills t cells by some pathways 10. Actinobacillus equuli actinobacillus ligniersii actinobacillus pleuropneumoniae.
M c healey, h h hwang, y y elsner, and a v johnston department of animal, dairy and veterinary sciences, college of agriculture, utah state university, logan 843225600. Actinobacillus pleuropneumoniae disease and serology. List of bacteria and similar organisms presenting at the. In immunodiffusion tests, hh group bacteria had at least two. Actinobacilli are usually transmitted via the aerosol route or by close contact. The present study reports the first isolation of actinobacillus seminis from a goat in brazil. It was first isolated in the united kingdom in 1991 and in one survey was found to be present in the semen of 19% of infertile rams. Frequency of outbreaks increased with the industrialization of swine production. Overview of actinobacillosis generalized conditions. Dec 21, 2019 download citation on researchgate actinobacillus seminis isolated from cattle the organism commonly referred to as actinobacillus. Actinobacillus actinomycetemcomitans is a typical cause of periodontitis but it may also be related to systemic infections and arterial plaques.
The major pathogenic actinobacillus species in veterinary medicine are presented in table 20. Actinobacillus pleuropneumoniae actinobacillus suis actinobacillus capsulatus actinobacillus equuli actinobacillus hominis actinobacillus lignieresii actinobacillus salpingitidis actinobacillus seminis actinobacillus ureae actinobacillus sp. The organisms can also gain entry through breaks in the skin. So far, only low levels of genetic relatedness with actinobacillus seminis and currently recognized members of the family pasteurellaceae have been detected. This short communication describes a retrospective analysis of bacterial isolates obtained between 1994 and 1996 in south wales from ejaculates of 63 rams that were considered by. Pdf clinical and pathological changes in rams experimentally. Actinobacillus lignieresi article about actinobacillus. Pcr methods for rapid identification and characterization.
Emended description of porcine pasteurella aerogenes, pasteurella mairii and actinobacillus rossii. Isolation and characterization of actinobacillus seminis strains from. Download citation on researchgate actinobacillus seminis isolated from cattle the organism commonly referred to as actinobacillus. Actinobacillosis refers to a group of diseases caused by gramnegative coccobacilli in the genus actinobacillus. Actinobacillus ureae article about actinobacillus ureae. Actinobacillus pleuropneumoniae app causes respiratory disease in swine throughout the world. However, the most common causative agents of these infections are brucella ovis, actinobacillus seminis, and histophilus somni.
Files are available under licenses specified on their description page. Twenty rams of age 18 to 24 months were infected by intraepididymal inoculation of a. Mar 21, 20 infectious ovine epididymitis results in substantial economic losses worldwide due to reproductive failure and culling of breeders. Clinical infections are usually unresponsive to treatment, and because infected animals are of considerable financial value, economic losses.
The bacteria are facultatively anaerobic or aerobic, capable of fermenting carbohydrates without production of gas, and of reducing nitrates. These pathogen safety data sheets, regulated under workplace hazardous materials information system whmis legislation, are produced for personnel working in the life sciences as quick safety reference material relating to infectious microorganisms. Clinical infections are usually unresponsive to treatment, and because infected animals are of considerable financial value. Fertility testing of rams nsw department of primary. Actinobacillus seminis as a cause of abortion in a uk. Actinobacillus m a taxonomic genus within the family pasteurellaceae several gramnegative bacteria, some of which are pathogens. Pdf actinobacillus seminis as a cause of abortion in a. Actinobacillus seminis infection in sheep in the republic of south africa. It is a gramnegative, nicotinamide adenine dinucleotide nadindependent, non. Actinobacillus seminis infection of the head of epididymis of a 4yearold suffolk ram. Semen examination does have a role in artificial insemination programs or in flocks where single sire joining groups are used. Pdf sheep epididymitis by actinobacillus seminis in the. Small ruminant research elsevier small ruminant research 21 1996 227231 actinobacillus seminis as a cause of ram infertility in kenya k mbaia, s.
Actinobacillus seminis an overview sciencedirect topics. Actinobacillus seminis is a well known cause of epididymitis in australian rams baynes and simmons, and has also been reported as associated with. Looking for a protein specific to this species, we determined the protein profile of subcellular fractions of a. The aim of this study was to develop a multiplex pcr assay for simultaneous detection of brucella ovis, actinobacillus seminis, and. In the outbreak, both young unmated rams and mature breeding rams were affected. Four of the rams had abnormal semen and three were also infertile. Actinobacillus suislike organism how is actinobacillus. Isolation of actinobacillus seminis from rams in the. Actinobacillus seminis was isolated from the semen of five rams on four farms.
Although there are 22 different bacterial species in this genus, only four a pleuropneumoniae, a suis, a equuli, and a lignieresii are frequently associated with disease in animals. By using welldefined techniques under optimum conditions it is possible adequately to define the biochemical characteristics of typical a. Speciesspecific multiplex pcr for the diagnosis of brucella ovis, actinobacillus seminis, and histophilus somni infection in rams by valeria s moustacas, teane ma silva, luciana f costa, mariana n xavier, custodio a carvalho, erica a costa, tatiane a paixao and renato l santos. Most species are nonhemolytic on blood agar, however colonies of actinobacillus suis on sheep blood agar is always surrounded by a clearly marked zone of hemolysis. Actinobacillus seminis as a cause of abortion in a uk sheep flock article pdf available in the veterinary record 14417. Murray pr, baron ej, jorgensen jh, pfaller ma, tenover fc, yolken hy eds 2. Pdf sheep epididymitis by actinobacillus seminis in the state of.
An outbreak of epididymitis and orchitis associated with actinobacillus seminis infection is reported. Infectious ovine epididymitis results in substantial economic losses worldwide due to reproductive failure and culling of breeders. Speciesspecific multiplex pcr for the diagnosis of brucella ovis. Mbiuki department of clinical studies, university of nairobi, nairobi, kenya b department of veterinary pathology, microbiology and parasitology, university of nairobi, nairobi, kenya accepted 29 november. Actinobacillus seminis as a cause of ram infertility in kenya. All structured data from the file and property namespaces is available under the creative commons cc0 license. Simone oliveira, advisor chair department of veterinary medicine of population abstract the early detection of pigs subclinically infected with a.
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